Use of aerobic microorganisms in aqueous systems to determine the biodegradation rate of materials. The test mixture consists of an inorganic culture medium, test material (the sole source of carbon and energy) with an organic carbon concentration ranging from 100 mg/L to 2 000 mg/L, and a suspension of activated sludge or compost or activated soil. of the culture medium. This mixture is incubated with stirring in a respirometer in a sealed flask for a period of not more than 6 months. Biochemical oxygen demand (BOD) was measured by absorbing the released carbon dioxide with a suitable absorber above the flask. The level of biodegradation is determined by the ratio of Biochemical Oxygen Demand (BOD) to Theoretical Oxygen Demand (ThOD), expressed as a percentage.